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Time (min) 0 - 38 38 - 50 50 - 60 Mobile phase A (per cent VV) 100 100 0 0 Mobile phase B (per cent VV) 0 0 100 100 Mobile phase : Time (min) 0 - 21 21 - 22 22 - 31 Mobile phase A (per cent VV) 100 100 0 0 Mobile phase B (per cent VV) 0 0 100 100 Flow rate: 1 mlmin.
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